Answers to this question can be found on bltadwin.ru give you a short summary: Faster way might be to use the parallel-fastq-dump, as suggested in this answer.I never tested that tool though, my own experience is that prefetch is more stable than fastq-dump command.. In that same post it seems that the Japanese mirror seems faster, don't know if that is still valid. · This will bltadwin.ru files of the run ERR from the ENA, or failing that, downloads bltadwin.ru file from the Amazon AWA Open Data Program and then converts to FASTQ, or failing that use NCBI prefetch to download and convert that to FASTQ. This will bltadwin.ru files of the run ERR from the ENA, or failing that, downloads bltadwin.ru file from the Amazon AWA Open Data Program and then converts to FASTQ, or failing that use NCBI prefetch to download and convert that to FASTQ. Kingfisher will do the least effort to convert a downloaded file into one of the formats specified in --output-format-possibilities which is fastq.
SeqSphere+ can be used to download FASTQ files from NCBI Sequence Read Archive (SRA). Invoke the function Tools | Download FASTQ from SRA to open a dialog window and enter or import the NCBI accessions that should be downloaded. The following types of accessions are supported (NCBI, EMBL-EBI, DDBJ). 2) Ftp download As far as I know, there is no way of directly accessing fastq files from NCBI. However, there is a ftp server which can be accessed using wget or a browser. Having said that, this still leaves the problem of converting sra files into fastq. Entrez Direct by default will download uncompressed data so you will end up spending more time downloading a larger file instead of downloading a smaller, compressed file from FTP more quickly. If you were to use Entrez Direct for this purpose, I'd not bother with a bash script and use epost to first post the entire list of accessions and then.
SeqSphere+ can be used to download FASTQ files from NCBI Sequence Read Archive (SRA). Invoke the function Tools | Download FASTQ from SRA to open a dialog window and enter or import the NCBI accessions that should be downloaded. Click on the Filtered Download button. Select available download format and click Download link. Aligned sequences example. Open the selected run in the Run Browser. Click the Alignment tab. Select available download format in pull-down menu and click on Screen or File button to output the run to the screen or into a file. National Center for Biotechnology Information; U.S. National Library of Medicine; National Institute of Health; United States Department of Health and Human Services; bltadwin.ru: The U.S. Government's Official Web Portal.
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